GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化

doi:10.11843/j.issn.0366-6964.2013.09.009

Abstract

Abstract:

To study the localization of GHR and IGF-IR and the expression of GHR mRNA and IGF-IR mRNA in uterus of Jining Grey goats during postnatal development. Real-time flourescence quantitative PCR was used to detect the expression pattern of GHR mRNA and IGF-IR mRNA, and SABC method was applied to research the distribution of positive cells of GHR and IGF-IR in the uterus. GHR and IGF-IR were mainly localized in the cytoplasm of glandular epithelium, stromal cells, endothelium and smooth muscle cells of the uterus, occasionally in the nuclei. The changed trends on the positive cells of GHR and IGF-IR were similar with that on the expression of its mRNA. Although the expression of IGF-IR was less than that of GHR, the expression of GHR and IGF-IR both reached its peak on the puberty (D60) and the sexual maturity (D120)（P<0.01）, Then decreased and remained at a relatively stable level. GHR and IGF-IR play an important role in the growth of the uterus, and the puberty and the sexual maturity may be the fastest period of the uterus during postnatal development.

CLC Number:

S827
S811.2

TONG Hai-ni, BAI Shu, WANG Shu-ying, HUANG Li-bo,SHI yun-zhi, HOU Yan-meng. Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1392-1399.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2013.09.009

https://www.xmsyxb.com/EN/Y2013/V44/I9/1392

References

［1］贾斌，席继峰，张苏云，等.绵羊皮肤中GHR、IGF-I和IGF-IR基因表达的发育性变化及品种特点［J］.遗传，2006，28(9)：1078-1082.

［2］SCANES C G, PROUDMAN J A, RADECKI S V. Influence of continuous growth hormone insulin-like growth factor I administration in adult female chickens ［J］.Gen Comp Endocr,1999, 114: 315-323.

［3］SPAGNOLI A, ROSENFELD R G. The mechanisms by which growth hormone brings about growth. The relative contributions of growth hormone and insulin-like growth factors ［J］. Endocrinol Metab Clin North Am, 1996, 25(3):615-631.

［4］马晓，胡毅，熊钢，等.生长激素受体及其基因研究新进展［J］.湖南农业大学学报(自然科学版)，2010，36（1）：1-2.

［5］BHAUMICK B, BALA R M, HOLLENBERG M D. Somatomedin receptor of human placenta: solubilization, photolabeling, partial purification and comparison with insulin receptor ［J］. Proc Natl Acad Sci USA, 1981, 78:4279-4283.

［6］邝月媚. 斜带石斑鱼胰岛素样生长因子受体IGF-IR cDNA的分子克隆和序列分析以及表达研究［D］.广州：中山大学，2004:6.

［7］蔡泉. 济宁青山羊品种资源调查［J］.中国草食动物，2008，28（6）：64.

［8］孙荪，朱尚权. 生长激素的结构和功能［J］. 国外医学生理、病理科学与临床分册，1999，19(1)：5-9.

［9］NUTTALL R K, KENNEDY T G. Epidermal growth factor and basic fibroblast growth factor increase the production of matrix metalloproteinases during in vitro decidualization of rat endometrial stromal cells ［J］. Endocrinology, 2000, 141(2):629-636.

［10］GREEN D R, EVAN G I. A matter of life and death［J］. Cancer Cell, 2002, 1(1):19-30.

［11］PORTIER C J. Endocrine dismodulation and cancer［J］. Neuro Endocrinol Lett, 2002, 23(Suppl 2): 43-47.

［12］NOEL S, LLERMAN A, JOHNSON G A, et a1. Ovine placental lactogen specifieially bands toendometrial glands of the ovine uterus ［J］. Biol Reprod,2003, 68: 772-780.

［13］SBRACIA M, SCARPELLINI F, POVERINI R, et al. Immunohistochemical localization of the growth hormone in human endomemum and deciduas ［J］. Reprod Immunol, 2004, 51(2):112-116.

［14］ILKBAHAR Y N, WU K, THORDARSON G. Expression and distribution of messenger ribonucleic acids for growth hormone (GH) receptor and GH-binding protein in mice during pregnancy ［J］. Endocrinology, 1995, 136(2): 386-392.

［15］徐金先，夏东，赵茹茜，等. 猪下丘脑和垂体中生长激素受体、胰岛素样生长因子I型受体的发育性变化［J］.遗传学报，2004，31(5)：495-501.

［16］KLEMPT M, BINGHAM B, BREIER B H. Tissue distribution and ontogeny of growth hormone receptor messenger ribonucleic acid and ligand binding to hepatic tissue in the midgestation sheep fetus ［J］. Endocrinology, 1993, 132(3): 1071-1077.

［17］RUTANEN E M. Insulin-like growth factors and insulin-like growth factor binding proteins in the endometrium. Effect of intrauterine levonorgestrel delinery［J］. Hum Reprod, 2000,15(Suppl 3): 173-184.

［18］LEROY F, GALAND P, CHRETIEN S. The mitogenic action of ovarine hormones on the uterine and the vaginal epithelium during the oestrous cycle in the rat: A radioutographic study［J］. J Endocrinol, 1969, 45:441.

［19］仓明，张东，旭日干. 胰岛素样生长因子及其受体mRNAs在绵羊生殖系统中的表达和分布［J］. 中国兽医科学，2007，(10): 876-881.

［20］QUAMBY V E, KORACK K S. The influence of 17 β-estradiol on patterns of cell division in the uterus［J］. J Endocrinol, 1984, 114:694.

［21］IRWIN J C, DELAS FUENTES L, GIUDICE L C. Growth factors and decidualization in vitro［J］. Ann N Y C Acad Sci, 1994, 734:737.

［22］GIUDICE L C, DSUPIN B A, JIN I H, et al. Differential expression of messenger ribonucleic acids encoding insulin-like growth factors and their receptors in human uterine endometrium and deciduas ［J］. J Clin Endocr Metab,1993, 76: 1115-1122.

［23］JIN H Y, WANG Z N. Insulin-like growth factors and their receptors in human fallopian tube in reproductive-age women ［J］. Fert Steril, 2001, 75(5): 1037-1038.

［24］WATHES D C, REYNOLDS T S, ROBINSON R S, et al. Role of insulin-like growth factor system in uterine function and placental development in ruminants［J］.Dairy Sci, 1998, 81:1778-1789.

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	MENG Qiuchi, LU Guangyu, CHEN Dingshuang, LIN Yaqiu, WANG Ruilong, ZHONG Chaosong, WANG Yong, LIU Wei, WANG Youli, LI Yanyan, LI Zhixiong. Goats GPR35 Gene Expression Characteristics Analysis and the Effect on Subcutaneous Fat Cell Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4993-5007.
[4]	HE Zhicheng, QIN Xiaochen, Lü Yongqiang, LI Xiujin, BIAN Huilong, LUO Jun, LI Cong. Multiple Stepwise Regression Analysis and Growth Curve Fitting of Body Measurement Traits in Saanen Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5301-5311.
[5]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[6]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[7]	LIU Jie, XU Xiangping, DENG Ming, ZOU Xian, JIANG Shengwei, LIU Dewu, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Effect of miR-144-5p Targeting WNT5a on the Proliferation and Apoptosis of Goat Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2421-2435.
[8]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[9]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[10]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[11]	WANG Li, GUO Yaru, ZHANG Junmei, LEI Mingkai, WANG Zhenguo, ZHANG Chunxiang, REN Youshe. Effect of 1,25(OH)₂D₃ and VDR Knockout on the Expression of β-defensins Family in the Caprine Epididymal Caput Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1990-2000.
[12]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[13]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.
[14]	DAN Yixin, YANG Lu, XIANG Hua, ZHANG Huanrong, REN Yupeng, YANG Falong, HE Honghong, ZHU Jiangjiang. Effects of BIRC5 on the Cycle and Apoptosis of Goat Testis Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1511-1524.
[15]	YE Junning, DENG Ming, XUE Huiwen, LIU Guangbin, ZOU Xian, SUN Baoli, GUO Yongqing, LIU Dewu, LI Yaokun. Identification and Analysis of mRNA and lncRNA Affecting Goat Fetal Muscle Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 989-1002.

Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments